Journal: BMC Cancer

Article Title: The marine triterpene glycoside frondoside A induces p53-independent apoptosis and inhibits autophagy in urothelial carcinoma cells

doi: 10.1186/s12885-017-3085-z

Figure Lengend Snippet: Effect of p53 silencing on cytotoxicity of FrA. a , b Western blotting analysis of p53 expression in RT112 cells treated with FrA for 48 h ( a ) or transfected with p53 siRNA versus cells transfected with scrambled siRNA ( b ). c Viability of transfected cells treated with FrA or Cis for 48 h. Cell viability was analyzed by flow cytometry analysis using annexin-V-FITC/PI double staining. Viable cells appearing in the lower left quadrant were quantified using the Cell Quest Pro software. d , e Effect of pifithrin-α (Pif-α)–an inhibitor of p53 activity–on the cytotoxic activity of FrA and Cis in nontransfected RT112 cells. Cells were pretreated with 40 μM of Pif-α for 30 min and then cotreated with FrA ( d ) or Cis ( e ) for 48 h. Cell viability was determined using MTT-assay

Article Snippet: The human urothelial cancer cell lines RT4 (p53 wild type), HT-1197 (p53 wild type), TCC-SUP (mutant p53), T-24 (mutant p53), were purchased from ATCC (Manassas, VA, USA) [ ].

Techniques: Western Blot, Expressing, Transfection, Flow Cytometry, Double Staining, Software, Activity Assay, MTT Assay

Journal:

Article Title: The R1 component of mammalian ribonucleotide reductase has malignancy-suppressing activity as demonstrated by gene transfer experiments

doi:

Figure Lengend Snippet: Information about cell lines

Article Snippet: Cells were obtained from subconfluent cultures, and colonies were scored 14–21 days later ( 20 ). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Designate Parental line Related characteristics and transgene expression Reference or source BHK ATCC Ψ2 Retroviral packaging ATCC PA317 Retroviral packaging ATCC PA/SHD PA317 Packaging control virus LXSHD This study PA/mR1 PA317 Packaging SHD/mR1 * virus This study SC2 L60 R2 is in excess as compared to R1 McClarty et al . ( 6 ) SC2/SHD SC2 his D † This study SC2/mR1 SC2 mR1, his D † This study CIRAS-1 10T½ T24 H- ras Egan et al . ( 25 ) C1/SHD CIRAS-1 his D † , T24 H- ras This study C1/mR1 CIRAS-1 mR1 * , his D † , T24 H- ras This study C1/mR2 CIRAS-1 mR2 * , T24 H- ras Fan et al . ( 20 ) C1/mR2/SHD C1/mR2 his D † , mR2 * , T24 H- ras This study C1/mR2/mR1 C1/mR2 mR1, his D † , mR2 * , T24 H- ras This study C1/mR2a/SHD C1/mR2a ‡ his D † , mR2 * , T24 H- ras This study C1/mR2a/mR1 C1/mR2a ‡ mR1 * , his D † , mR2 * , T24 H- ras This study ras-3 (or R-3) 10T½ T24 H- ras Taylor et al . ( 26 ) ras-3/SHD ras-3 his D † , T24 H- ras This study ras-3/mR1 ras-3 mR1 * , his D † , T24 H- ras This study RMP-6 10T½ T24 H- ras , c- myc , mutant p53 Taylor et al . ( 26 ) RMP/SHD RMP-6 his D † , T24 H- ras , c- myc , mutant p53 This study RMP/mR1 RMP-6 mR1 * , his D † , T24 H- ras , c- myc , mutant p53 This study Colo 320HSR Human colon carcinoma ATCC Colo/SHD Colo 320HSR his D † This study Colo/mR1 Colo 320HSR mR1 * , his D † This study NIH 3T3 ATCC N/ ras NIH3T3 T24 H- ras , neo † This study N/ ras &ASR1 NIH3T3 R1 antisense, T24 H- ras , neo † This study Open in a separate window ATCC, American Type Culture Collection.

Techniques: Expressing, Mutagenesis

Journal:

Article Title: The R1 component of mammalian ribonucleotide reductase has malignancy-suppressing activity as demonstrated by gene transfer experiments

doi:

Figure Lengend Snippet: Information about cell lines

Article Snippet: Cells were obtained from subconfluent cultures, and colonies were scored 14–21 days later ( 20 ). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Designate Parental line Related characteristics and transgene expression Reference or source BHK ATCC Ψ2 Retroviral packaging ATCC PA317 Retroviral packaging ATCC PA/SHD PA317 Packaging control virus LXSHD This study PA/mR1 PA317 Packaging SHD/mR1 * virus This study SC2 L60 R2 is in excess as compared to R1 McClarty et al . ( 6 ) SC2/SHD SC2 his D † This study SC2/mR1 SC2 mR1, his D † This study CIRAS-1 10T½ T24 H- ras Egan et al . ( 25 ) C1/SHD CIRAS-1 his D † , T24 H- ras This study C1/mR1 CIRAS-1 mR1 * , his D † , T24 H- ras This study C1/mR2 CIRAS-1 mR2 * , T24 H- ras Fan et al . ( 20 ) C1/mR2/SHD C1/mR2 his D † , mR2 * , T24 H- ras This study C1/mR2/mR1 C1/mR2 mR1, his D † , mR2 * , T24 H- ras This study C1/mR2a/SHD C1/mR2a ‡ his D † , mR2 * , T24 H- ras This study C1/mR2a/mR1 C1/mR2a ‡ mR1 * , his D † , mR2 * , T24 H- ras This study ras-3 (or R-3) 10T½ T24 H- ras Taylor et al . ( 26 ) ras-3/SHD ras-3 his D † , T24 H- ras This study ras-3/mR1 ras-3 mR1 * , his D † , T24 H- ras This study RMP-6 10T½ T24 H- ras , c- myc , mutant p53 Taylor et al . ( 26 ) RMP/SHD RMP-6 his D † , T24 H- ras , c- myc , mutant p53 This study RMP/mR1 RMP-6 mR1 * , his D † , T24 H- ras , c- myc , mutant p53 This study Colo 320HSR Human colon carcinoma ATCC Colo/SHD Colo 320HSR his D † This study Colo/mR1 Colo 320HSR mR1 * , his D † This study NIH 3T3 ATCC N/ ras NIH3T3 T24 H- ras , neo † This study N/ ras &ASR1 NIH3T3 R1 antisense, T24 H- ras , neo † This study Open in a separate window ATCC, American Type Culture Collection.

Techniques: Expressing, Mutagenesis

Journal:

Article Title: The R1 component of mammalian ribonucleotide reductase has malignancy-suppressing activity as demonstrated by gene transfer experiments

doi:

Figure Lengend Snippet: Analysis of Myc-tagged R1 expression from pSHD/mR1 transiently transfected BHK cells by the indirect immunofluorescence assay (A), and from the stable retroviral packaging cell line PA/mR1 by radioimmunoprecipitation (B). Anti-Myc epitope antibody 9E10 was used for both assays.

Article Snippet: Cells were obtained from subconfluent cultures, and colonies were scored 14–21 days later ( 20 ). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Designate Parental line Related characteristics and transgene expression Reference or source BHK ATCC Ψ2 Retroviral packaging ATCC PA317 Retroviral packaging ATCC PA/SHD PA317 Packaging control virus LXSHD This study PA/mR1 PA317 Packaging SHD/mR1 * virus This study SC2 L60 R2 is in excess as compared to R1 McClarty et al . ( 6 ) SC2/SHD SC2 his D † This study SC2/mR1 SC2 mR1, his D † This study CIRAS-1 10T½ T24 H- ras Egan et al . ( 25 ) C1/SHD CIRAS-1 his D † , T24 H- ras This study C1/mR1 CIRAS-1 mR1 * , his D † , T24 H- ras This study C1/mR2 CIRAS-1 mR2 * , T24 H- ras Fan et al . ( 20 ) C1/mR2/SHD C1/mR2 his D † , mR2 * , T24 H- ras This study C1/mR2/mR1 C1/mR2 mR1, his D † , mR2 * , T24 H- ras This study C1/mR2a/SHD C1/mR2a ‡ his D † , mR2 * , T24 H- ras This study C1/mR2a/mR1 C1/mR2a ‡ mR1 * , his D † , mR2 * , T24 H- ras This study ras-3 (or R-3) 10T½ T24 H- ras Taylor et al . ( 26 ) ras-3/SHD ras-3 his D † , T24 H- ras This study ras-3/mR1 ras-3 mR1 * , his D † , T24 H- ras This study RMP-6 10T½ T24 H- ras , c- myc , mutant p53 Taylor et al . ( 26 ) RMP/SHD RMP-6 his D † , T24 H- ras , c- myc , mutant p53 This study RMP/mR1 RMP-6 mR1 * , his D † , T24 H- ras , c- myc , mutant p53 This study Colo 320HSR Human colon carcinoma ATCC Colo/SHD Colo 320HSR his D † This study Colo/mR1 Colo 320HSR mR1 * , his D † This study NIH 3T3 ATCC N/ ras NIH3T3 T24 H- ras , neo † This study N/ ras &ASR1 NIH3T3 R1 antisense, T24 H- ras , neo † This study Open in a separate window ATCC, American Type Culture Collection.

Techniques: Expressing, Transfection, Immunofluorescence